Abstract
Sensitive and easy applicable analysis of Quercetin molecules was executed whereby solid phase extraction (SPE) and molecular spectrophotometric determination. For the determination of quercetin, an accurate, economical, time-saving and a new environmentally friendly method has been developed with a simple spectrophotometer device available in every laboratory. As a solid-phase sorbent, a new polymer nanocomposite has been synthesized and characterized by Fouirer Transform Infrared Spectroscopy (FT-IR) and Scanned Electron Microscopy (SEM). Experimental solid phase extraction variables have been investigated and optimized step by step, such as pH, adsorption time and desorption conditions, etc. According to optimization results, desorption of quercetin molecules was carried out by 2-propanol solvent and absorbance of eluent solutions were measured as systematic at 255 nm and at 370 nm. However, the best results were obtained at 370 nm wavelength. Therefore, all measurements were made at 370 nm. (LOD) means that the detection limit and (LOQ), the quantitation limit values for quercetin were calculated as 5.97 ng mL-1and 17.91 ng mL-1, respectively. Standard addition and recovery experiments were performed as an indicator of the accuracy of the method. The method developed was effectively conducted to model solutions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.