Abstract
Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of "conditioned medium" for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.
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