Synthesis of N, S co-doped carbon quantum dots (N,S-CQDs) for sensitive and selective determination of mercury (Hg2+) in Oreochromis niloctus (Tilapia fish)

Abstract

This study reported the synthesis of green-emitting N,S-doped carbon quantum dots (N,S-CQDs) through a one-step, facile, and fast hydrothermal technique using citric acid, glutamine, and Na2S as the carbon, nitrogen, and sulphur sources respectively. The fluorescence efficiency of the developed nanosensor was investigated by varying the duration of synthesis (4, 5, and 6 h). Characterization of the N-CQDs was achieved using Fourier-Transform Infra-red Spectroscopy (FTIR), High-resolution transmission electron microscope (HRTEM), ultraviolet–visible spectroscopy (UV–Vis), and X-Ray diffraction analysis (XRD). The as-synthesized N,S-CQDs are small (average size of 3.45 ± 0.86 nm), spherical, and displayed green emission (535 nm) with a fluorescent quantum yield (QY) of 10.35%. FTIR analysis reveals carboxylic, hydroxyl, and conjugated amide functional groups. The fluorometric study showed that the developed nanosensor was selective and sensitive towards Hg2+ ions in the midst and absence of interfering ions with a detection limit (LOD) of 28.9 nM. The static quenching mechanism was proposed based on lifetime and UV results. Furthermore, it was used as a nanoprobe for Hg2+ determination in Oreochromis niloctus with recoveries of 96.7–108.6% (RSD < 4.1%), indicating the as-synthesized N,S-CQDs have potentials as a nanoprobe for Hg2+ determination in sea foods.