Abstract
Magnetite nanoparticles (NPs) were synthesized using a cost-effective co-precipitation method. Magnetite NPs were encapsulated with silica via the modified Stober Method. Tetra ethyl ortho silicate (TEOS) was hydrolyzed and condensed with ethanol and H2O solution. Stable and biocompatible NPs were synthesized for biomedical applications such as bioseparation. This study expresses the NPs that can potentially be used in the bioseparation of toxic protein isolations and targeted drug delivery. X-ray diffraction verified the phase pattern having crystals like Fd-3[Formula: see text]m cubic space. Scanning electron microscopy (SEM) images identified the spherical-shaped NPs having size ranges from 15[Formula: see text]nm to 30[Formula: see text]nm for magnetite NPs and 20–40[Formula: see text]nm for silica-coated magnetite NPs. Fourier transform infrared spectroscopy (FTIR) confirmed the bond spectrum peak at 549[Formula: see text][Formula: see text] and 562[Formula: see text][Formula: see text] for magnetite NPs and silica-coated magnetite NPs, respectively. UV–Visible analysis observed the band absorptions above 250[Formula: see text]nm for magnetite and above 300[Formula: see text]nm for silica-coated magnetite NPs. This research suggests an easy way to use silica-coated Magnetite NPs for bioseparation at room temperature.
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