Abstract
Molecularly imprinted polyacrylamide cryogels were synthesized with pending templates (bovine serums of different concentrations). As the serum concentrations increased in the monomer solutions, the resulting cryogels could adsorb and deplete more proteins from serum samples. Due to the addition of vinyltriethoxysilane (VTEOS) in the prepolymerizing solutions, the polymers came as organic–inorganic hybrid materials. It endued the silica-modified amphoteric polyacrylamide cryogels with improved mechanical strengths. Scanning electron micrography (SEM), Infrared (IR) spectrometry, thermogravimetry-differential thermal analysis (TG-DTA), and X-ray photoelectron spectroscopy (XPS) were carried out to characterize these macroporous polymers. Amphoteric cryogels proved to be favorable materials recognizing and binding proteins. When used as liquid chromatography stationary phases, they were capable of simultaneously adsorbing various serum proteins. Electrophoresis showed that abundant proteins were gradually depleted by the cryogels prepared from increased ratios of bovine serums in the monomer solutions. As abundant proteins are always imprinted first, this sample per se imprinting method provides an effective and convenient way to deplete abundant proteins from complex samples such as serums, meanwhile concentrating and collecting scarce species therein.
Highlights
Imprinted polymers (MIPs) are synthesized to achieve specific interactions and mutual responses between the polymeric matrices and the related templates [1,2]
In many aspects the non-imprinted polymer (NIP) is similar to the molecularly macropores around
An optionally allocated mixture of AM, BisAM, acrylic acid and diallylamine is not possibly an optimal20choice to imprint any certain template, it is effective for the imprinting of various proteins
Summary
Imprinted polymers (MIPs) are synthesized to achieve specific interactions and mutual responses between the polymeric matrices and the related templates [1,2]. Those stable polymeric materials possess amazing properties and functions by bearing predetermined exclusive binding sites against certain target molecules (templates). Ice crystal squeezing effects influence skeleton structures and manipulate the stacking layout of chemical groups in cryogels Besides conventional functions such as protein recognition [18,19,20,21], purification/separation [9,22,23,24], or depletion [25], cryogels could be prepared to act as catalysts [26] and fluorescent materials [27]. Sample understanding seems unnecessary, because it is an efficient and low-cost method achieved by feeling free to perform molecular imprinting against sample heterogeneities
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