Abstract
Hydrophobic mesoporous silica nanowires were synthesis and then employed as support for immobilization of lipase from Candida antarctica via covalent bonding (CALB@MSW). The parameters were optimized and the optimum conditions were as follows: GA concentration 5.5 wt.%, activation time 60 min and CALB concentration 4 mg/mL. Under these conditions, the protein loading and specific activity of CALB@MSW were 138.3 mg/gsupport and 41.1 U/mgsupport, respectively. Compared with free CALB, CALB@MSW showed better thermal stability and pH stability. The maximum yield of biodiesel catalytic by CALB@MSW was 93.4 %. After reused 8 times, CALB@MSW still remained 95.75 % initial activity showing better stability than free CALB.
Highlights
Lipase possesses very broad substrate specificity and has extensively utilized in chemical and pharmaceutical industry to produce high value-added chemicals, biosensors and drugs
Candida antarctica lipase B (CALB) was immobilized to Mesoporous silica nanowires (MSW) by covalent bond with GA as crosslinker. 100mg of MSW was suspended in 5 mL of phosphate buffer solution (PBS, 10 mM, pH 7.0) containing 0.2% GA
With the extension of activation time from 30 min to 90 min, the specific activity of CALB@ MSW increased first and decreased. This was because the short activation time resulted in insufficient amount of aldehyde groups on MSW and limited the loading amount of CALB leading to lower activity[7]
Summary
Lipase possesses very broad substrate specificity and has extensively utilized in chemical and pharmaceutical industry to produce high value-added chemicals, biosensors and drugs. Many materials have explored as a support for immobilization, such as zeolite, resin, sol-gels, polymeric hydrogels, mesoporous carbon, organic microparticles and controlled pore glass[2]. Mesoporous silica nanowires (MSW) with three-dimensional (3D) network and hierarchical pore structures are an outstanding representative. Their applications in catalysis have aroused extensive research interest due to their considerable advantages, including high surface area, mechanical stability and favorable mass transport taking place in both reaction and sorption processes benefitting from the multimodal pore systems[4]. The MSW are used as a catalyst support for the immobilization of Candida antarctica lipase B (CALB) and tested for biodiesel production to explore their applicability. The optimum synthesis conditions of the catalyst were studied based on the yield of biodiesel
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
