Abstract
In an attempt to produce novel agronomic traits, a number of intraspecific somatic hybrid plants have been produced following leaf mesophyll protoplast fusion between S. tuberosum dihaploid clones PDH 40 (possessing good tuber shape and yield) and PDH 417 (possessing resistance to potato cyst nematode, G. pallida). PDH 417 protoplast-derived calli failed to regenerate plants using the described culture conditions preventing this parental type amongst the mass of regenerated fusion products. Initially, somatic hybrid plants were selected based on differential pigmentation in tuber sprouts and where possible on petal colour. Differential mobility of patatin bands in electrophoresed tuber extracts further confirmed hybridity
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