Abstract
A new approach towards the design of folic acid conjugated magnetic nanoparticles forenhancing their site specific intracellular uptake against a folate receptor overexpressingcancer cells is reported. Magnetite nanoparticles were prepared by coprecipitation from anFe3+ and Fe2+ solution followed by surface modification with 2-carboxyethyl phosphonic acid to formcarboxyl group terminated nanoparticles. Then folic acid and fluorescein isothiocyanate(FITC) were conjugated with carboxylic acid functionalized magnetite nanoparticles using2,2′-(ethylenedioxy)-bis-ethylamine. These folate-conjugated nanoparticles were characterizedin terms of their size by dynamic light scattering (DLS) and transmission electronmicroscopy (TEM). Surface functional groups and surface composition were analyzed byFourier transform infrared (FTIR) spectroscopy and x-ray photoelectron spectroscopy(XPS), respectively. Vibration sample magnetometry (VSM) measurements showed thesuperparamagnetic nature of the particles at room temperature. Folate-conjugatedmagnetic nanoparticles are noncytotoxic and receptor mediated internalization by HeLaand B16 melanoma F0 cancer cells was confirmed by flow cytometry and confocalmicroscopy.
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