Synthesis of highly dispersed nanoscaled CoQ10 liposome by supercritical fluid

Abstract

The nanoscaled CoQ10 liposome were synthesized by Rapid Expansion of Supercritical Solutions (RESS). Transmission electron microscopy (TEM) was used to characterize the particle size of CoQ10 liposome, and the UV spectrum was employed to evaluate the product׳s trapping efficiency. A series of CoQ10 liposome samples were synthesized by RESS under different pressures. The trapping efficiency of the CoQ10 liposome was up to 90% when the enduring pressure was at 200–300bar, but the trapping efficiency was distinctly decreased when the enduring pressure is lower than 200bar or higher than 300bar. The high resolution TEM images exhibited that the particle size of CoQ10 liposome was only 20–40nm, probably because CO2 molecules act as the membranes between the CoQ10 liposome particles in this process, which prevent the agglomeration of liposome with each other.