Synthesis of Glycosides of Resveratrol, Pterostilbene, and Piceatannol by Glucosyltransferase from Phytolacca americana Expressed in Bacillus subtilis and their Chemopreventive Activity Against Cancer, Allergic, and Alzheimer’s Diseases

Abstract

Resveratrol was glucosylated to its 3- and 4'-β-glucosides by the glucosyltransferase PaGT3 from Phytolacca americana expressed in Bacillus subtilis. PaGT3 glucosylated pterostilbene to its 4'-β-glucoside. Piceatannol was converted into its 4'-β-glucoside by PaGT3. Resveratrol 3-β-glucoside showed the inhibitory action of adhesion, migration, and invasion of KB cells. The resveratrol 3-β-glucoside would act as a chemopreventive agent against KB cells. Piceatannol 4'-β-glucoside showed the strongest inhibitory activity among the glycosides toward histamine release from rat peritoneal mast cells. The 4'-β-glucoside of piceatannol would be a potential therapeutic agent for allergic disease. Pterostilbene 4'-β-glucoside showed high phosphodiesterase (PDE) inhibitory activity. The 4'-β-glucoside of pterostilbene could be a potential chemopreventive agent for Alzheimer’s disease.