Abstract
A cell-free system catalysing the synthesis of β-glucan from uridine-diphosphoglucose was prepared from Aureobasidium pullulans. The activity was stable in the presence of 1 M sucrose and 10 mM MgSO4. The polymer produced was insoluble in H2O or acetic acid (0.5 M) and soluble in NaOH (0.5 M). Several enzyme preparations containing β-glucanase activity degraded the polymer to various extents. Synthesis of the polymer was enhanced by the presence of cellobiose and bovine serum albumin, but not by NaF or ATP. A Lineweaver–Burke plot of enzyme activity versus substrate concentration revealed biphasic kinetics. The enzyme preparation was subject to partial activation by trypsin and chymotrypsin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
