Abstract
1. 1. Synthesis of fibroin has been elicited in the cultured large ampullate glands of the spider Nephila clavipes. 2. 2. A dramatic wave of protein synthesis ensues 60 min after stimulation, preceded by a wave of RNA synthesis. 3. 3. The glands in culture synthesize the full-size protein product. 4. 4. Both stimulus-elicited responses are expressed exclusively by the gland tissue. 5. 5. In addition to the luminar product, the secretory epithelium contains an array of lower molecular size peptides bearing a striking resemblance to those reported as products of translational pauses during the synthesis of silk in Bombyx mori.
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