Abstract
The biosynthesis of exocellular phosphomannan has been demonstrated with protoplasts of Hansenula holstii NRRL Y-2448. The protoplasts were produced by the snail-gut enzyme, and incubation of the protoplasts with labelled glucose and phosphate led to the formation of an exocellular product which was characterized by its hydrolytic properties at pH 2.5. The products of the hydrolyzed polymer had the same Chromatographie characteristics on Sephadex and on paper as the authentic phosphomannan added as carrier.
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