Abstract
Crude enzyme obtained from suspension cultures of Catharanthus roseus catalysed the coupling of vindoline and catharanthine to form dimeric indole alkaloids. Substantially improved yields were realized through the use of flavin mononucleotide and manganous ion in the reaction mixture. With the optimized conditions, ca 25% of the substrates were converted to 3′,4′-anhydrovinblastine.
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