Abstract
Clostridium thermoaceticum catalyzes the net synthesis of acetate from CO2 according to the following overall reaction 2CO2+8H→CH3COOH+2H2O (Barker and Kamen, 1945;Wood, 1952). Our earlier observation that intrinsic factor partially inhibits the incorporation of 14CO2 into acetate by cell-free extracts of C. thermoaceticum (Poston, et al., 1964) suggested that a vitamin B12 derivative might be involved (Ellenbogen, 1960). This suggestion was supported by the discovery that cell-free extracts convert the methyl moiety of Co-14CH3-cobalamin into the methyl group of acetate (Poston, et al., 1964) and by the recent demonstration ofLjungdahl, Irion, and Wood (1965) that Co-14-CH3-cobyric acid and Co-14CH3-5-methoxy-benzimidazoyl cobamide accumulate in cells of C. thermoaceticum following their exposure to 14CO2.The present study shows that extracts of C. thermoaceticum catalyze the de novo synthesis of Co-methyl cobalamin in the presence of pyruvate, coenzyme A, CO2, and reduced cobalamin.
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