Synthesis of chitosan succinate-g-amine functionalized mesoporous silica: Inorganic-organic nanohybrid for antibacterial assessment, antioxidant activity and pH-controlled drug delivery

Abstract

An innovative and proficient inorganic-organic nanohybrid was synthesized by using amine modified MCM-41 as an inorganic precursor combined with organic moiety, a derivative of chitosan i.e. chitosan succinate through amide bond. These nanohybrids can be used in diverse applications due to potential combination of desired properties of inorganic and organic components. The nanohybrid was characterized by FTIR, TGA, small angle powder XRD, zeta potential, particle size distribution, BET, proton NMR and 13C NMR techniques to confirm its formation. The synthesized hybrid was loaded with curcumin drug to check its potential application for controlled drug release, showing 80 % drug release in acidic medium (i.e. pH -5.0), while physiological pH -7.4 shows only 25 % release. The encapsulation efficiency of nanohybrid is 87.24 %. The results of antibacterial performances are demonstrated in terms of ZOI (zone of inhibition) which depicts that hybrid material shows better ZOI in gram negative (E. coli) than for gram positive (B. subtilis) bacteria. Nanohybrid was also tested for the antioxidant activity by using two different methods (DPPH and ABTS) radical scavenging methods. The ability of nano-hybrid to scavenge DPPH radicals was found 65 %, and ability to scavenge ABTS radicals was 62.47 %.