Synthesis of Chitosan-Silver Nanoparticle Composite Spheres and Their Antimicrobial Activities.

Erisna Mirda,Muslem Muslem,Trina Ekawati Tallei,Khairan Khairan,Aga Maulana,Muliadi Ramli,Ghazi Mauer Idroes,Nanda Earlia,Zulkarnain Jalil,Rinaldi Idroes

doi:10.3390/polym13223990

Abstract

Synthesis of silver nanoparticles–chitosan composite particles sphere (AgNPs-chi-spheres) has been completed and its characterization was fulfilled by UV–vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and zetasizer nano. UV–vis spectroscopy characterization showed that AgNPs-chi-spheres gave optimum absorption at a wavelength of 410 nm. The XRD spectra showed that the structure of AgNPs-chi-spheres were crystalline and spherical. Characterization by SEM showed that AgNPs-chi-spheres, with the addition of 20% of NaOH, resulted in the lowest average particle sizes of 46.91 nm. EDX analysis also showed that AgNPs-chi-spheres, with the addition of a 20% NaOH concentration, produced particles with regular spheres, a smooth and relatively nonporous structure. The analysis using zetasizer nano showed that the zeta potential value and the polydispersity index value of the AgNPs-chi-sphere tended to increase with an increased NaOH concentration. The results of the microbial activity screening showed that the AgNP-chi-Spheres with highest concentration of NaOH, produced the highest inhibition zone diameters against S. aureus, E. coli, and C. albicans, with inhibition zone diameters of 19.5, 18.56, and 12.25 nm, respectively.

Highlights

Silver nanoparticles have several unique features, including electronic properties, catalytic properties, optical properties [3], high surface energy, and less aggregation, in addition to several other physical and chemical properties which are not possessed by other metal nanoparticles [4]
[7] It is reported that silver nanoparticles could inhibit the growth of Gram-positive bacteria (Staphylococcus aureus and Bacillus sp.), Gram-negative bacteria (Escherichia coli, Proteus sp., Pseudomonas sp., Serratia sp., Klebsiella sp.), and pathogenic fungi (Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Candida albicans) [5]
The results show that the zeta potential value of silver nanoparticles–chitosan composite spheres increased with an increasing NaOH concentration

Summary

Introduction

The synthesis of nanoparticles (1–100 nm) has shown various advantages, including: minimizing material requirements, increasing activity due to increased surface area, and having unique chemicalphysical properties. Past studies indicate the wide synthesis of metal nanoparticles because they have superior biological, optical, electrical, catalytic activity, and antimicrobial properties [1,2]. Silver nanoparticles have been widely used in various fields such as photocatalyst, lithography, microelectronic biosensor materials, and pharmaceuticals. It can be used as an alternative to the use of a natural product as a medicinal substance which requires raw materials from biodiversity which are starting to become less available [5,6]. Silver nanoparticles are active against viruses such as the hepatitis B virus [8], Herpes Virus Type

Methods

Results

Conclusion