Abstract
Current situation demands more biocompatible and non-toxic nanostructures. Towards this the present study describes the synthesis of gold nanoparticle by direct reduction of tetrachloroauric acid with both D- and L-enantiomeric forms of the amino acid serine. The formation of nanoparticle was confirmed by examining different physical characteristics like surface plasmon resonance. Moreover, the retention of chirality of the reduced particle was also evident from circular dichroism spectroscopy experiment. In addition, the shielding of the nanoparticle by respective amino acid was confirmed by Scanning Electron Microscope and Energy Dispersive X-ray Analysis (SEM-EDAX). As D-serine is known to be active against Mycobacteria, examination of the biological activity of the D-serine protected particle was performed against the same. The result showed higher inhibitory activity of the particle against Mycobacterium smegmatis than D-serine alone. Thus the present study describes a new protocol for the synthesis of chiral gold nanoparticle that can be beneficial in enantioselective biological applications.
Highlights
The use of nanomaterial in biology is an emerging field with potentially wide range of applications, such as bioimaging, biosensing, gene delivery and in medicine [1,2]
Particle synthesized by 100 mM of both D-serine and L-serine was visualized under Transmission Electron Microscope (TEM) and the average size was found to be 52.8 ± 5.33 nm (Figure 1c) and 29.6 ± 4.78 nm, respectively (Figure 2c)
In this study we have described the synthesis of chiral gold nanoparticle (GNP) by direct reduction with both the enantiomeric forms of amino acid
Summary
The use of nanomaterial in biology is an emerging field with potentially wide range of applications, such as bioimaging, biosensing, gene delivery and in medicine [1,2]. Increased use of synthetic nanomaterials in biological application demands a more biocompatible, safe and effective nanostructure with less hazardous byproducts of synthesis reactions. In this context, besides classical methods of synthesis [3,4], methods of GNP synthesis using biocompatible agents as reducing as well as protection agents are gaining importance. Control of size and morphology of GNPs is of prime importance for optimizing its applications in biological fields. These properties of nanoparticle are completely dependent on the type and concentration of reducing agent used in the reaction. There are reports of synthesis of GNP by direct reduction with amino acids or capping of chemically synthesized GNP with the amino acids like tryptophan, lysine, aspartic acid, cysteine etc [10,11,12]
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