Abstract
Summary: During the adaptation of Pseudomonas aminovorans from growth on succinate to growth on trimethylamine, the following enzymes were synthesized in the lag phase before exponential growth on trimethylamine began: trimethylamine and dimethylamine mono-oxygenases, trimethylamine-N-oxide aldolase (demethylase), glutathione- and NAD-dependent formaldehyde dehydrogenase, dye-linked formaldehyde dehydrogenase, hydroxypyruvate reductase and N-methylglutamate dehydrogenase. Differential plots suggested that the rate of enzyme synthesis in the lag phase exceeded the rate of synthesis during exponential growth. The evidence suggests that the enzymes discussed are essential for growth on trimethylamine, while the NADPH-dependent N-methylalanine dehydrogenase is not involved.
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