Synthesis of CdTe quantum dots using Glutathione promoted in Vitro Interaction between Lysozyme and Flavanoids: An investigation through spectroscopic methods

Abstract

We investigated the interaction of four flavonoids in this study, such as 4′,5,7-trihydroxy-isofavonethe or 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (Genistein-GES), 5,7-Dihydroxy-2-phenyl-4H-1-benzopyran-4-one (Chrysin-CRS), 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxychromen-4-one (Quercetin-QRS) and 7-Hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (Daidzein-DIA), [0–5 × 10−6 M] and lysozyme without and the existence of the GSHCdTe quantum dots. Here, the spectrofluorimetric technique is used to investigate the interaction and quenching observations are rationalized in terms of a static quenching way. The binding constant (K) and the number of binding sites (n) are calculated based on the fluorescence quenching data. The affinities of flavonoids and lysozyme system enhance with smaller size quantum dots. The as-prepared GSH-capped QDs clearly reveal the linkage attractions of lysozyme and flavonoids enhanced with diminishing the quantum dots size and the interaction depends on physicochemical and environmental factors.