Abstract
<p class="Els-Abstract-text"><em>Capsicum frutescens</em> L. cv. Cakra Hijau is a local cultivar that has been widely cultivated in Indonesia due to the pungency. Pungent on <em>Capsicum</em> is generated by capsaicin compound encoded by <em>Pun1</em> gene. The sequences of <em>Pun1 </em>gene containing with two exons that located on the upstream and downstream, which are separated by introns in the middle. This study aimed to synthesis of cDNA of <em>Pun1</em> gene from isolated total mRNA using two primers: F1/R1 (<strong><em>F1 </em></strong>5’- ATG GCT TTT GCA TTA CCA TCA -3’; and <strong><em>R1 </em></strong>5’-CTT AGC TCG AAG TGC ATC TA-3’) to synthesis the exon-1 sequences and F2/R2 (<strong><em>F2 </em></strong>5’-GAA GGT GGC AGA AGA ATC AG-3’; and <strong><em>R2 </em></strong>5’-TTA GGC AAT GAA CTC AAG GA-3’) to synthesis the exon-2 sequences. The cDNAs resulted from RT-PCR were visualized on 1.5 % agarose gel electrophoresis. From this study we obtained a 1323 bp fragment cDNA.</p><p> </p><p><strong>Keywords: </strong><em>Capsicum frutescens</em><em> </em>L. cv<em>.</em>Cakra Hijau; <em>Pun1 </em>gene; synthesis of cDNA.</p>
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