Abstract
Poly(A) +RNA was isolated from Chlamydomonas reinhardtii cells grown autotrophically in ordinary air (low-CO 2 cells) and in air enriched with 4% CO 2 (high-CO 2 cells). In vitro translation products with the messenger RNA showed that the synthesis of the 37-kDa peptide was greater in low-CO 2 than in high-CO 2 cells. The immunoprecipitate produced by treating the translation products with antiserum against carbonic anhydrase (CA) from this alga was also 37 kDa. Comparison of radioactivities incorporated into these 37 kDA peptides suggested that CA induction by lowering atmospheric CO 2 concentration is regulated at the transcriptional level.
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