Abstract

This paper describes the capping reaction of 5′-phosphorylated oligoribonucleotides protected only by a tetrahydropyranyl group at each 2′-position. By use of the conventional capping agent PhSppm7GTMTrmM, m7G5pppGUUA and m7G5′pppGUAUUA were synthesized from the corresponding oligoribonucleotide 5′-monophosphate derivatives. Since the latter was obtained in low yield because of poor solubility of the partially protected pUUA in DMF, the oligomer component was converted into a phosphorimidazolidate species which was found to be soluble in DMF and allowed to react with a newly designed capping agent, ppm7GTMTrmM. This triphosphate bond formation proceeded smoothly to give m7G5′pppGUAUUA in good yield.

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