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Abstract
Abstract We have detected significant hemolytic C4 activity after transplant of normal guinea pig bone marrow into lethally irradiated guinea pigs genetically deficient in C4. Each of five C4-deficient guinea pigs received 640 rads total body irradiation. They were immediately injected intracardially with 93 to 200 × 106 allogeneic nucleated marrow cells from Albany strain normal guinea pigs. The recipients were followed by sequential white cell counts and titrations of total complement, C1, C4, and C2. C4 activity, initially undetectable, was present as early as 24 days after transplantation and reached maximum levels by 45 to 60 days. C4 protein was also identified by using monospecific anti-guinea pig C4 serum. Amounts of C4 were sufficient to restore classical pathway function. C1 activity, initially 2 S.D. below normal, rose dramatically to normal or greater than normal levels. C2 activity also increased. These data demonstrate that allogeneic donor stem cells protected the lethally irradiated C4-deficient recipients and these allogeneic radiation chimeras subsequently synthesize circulating functional C4.
Published Version
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