Abstract
Aurumacryl is an incomplete metal salt of poly(acrylic acid) that exhibits hemostatic activity and inhibits the growth of transplantable carcinomas in vivo. The samples of aurumacryl synthesized following the original technique are insufficiently soluble, which complicates the study of the mechanisms involved in their synthesis and underlying their cytotoxic effect. The aim of this work was to study the impact of the following factors on aurumacryl properties: the molecular weight of the polyacrylate polymer in a range between 2 and 1,000 kDa, the presence of a counterion H+ or Na+, the molar ratio of AuCl– to the polyacrylate polymer (1 : 5 and 1 : 10), the total concentration of the polyacrylate polymer during synthesis (0.1 and 3%), and the type of drying (lyophilization). By comparing the cytotoxicity of aurumacryl samples with significantly different molar ratio of gold ions to the polyacrylate polymer against human breast carcinoma cells (MCF-7), we established that the proportion of the polymer and its molecular weight in the sample do not affect the biological properties of the synthesized substance. Using UV spectroscopy, we revealed that the concentration of Au (III) ions in aurumacryl determines its cytotoxicity.
Highlights
IntroductionСопоставление цитотоксической активности полученных образцов аурумакрила со значительно различающимся соотношением ионов золота и полиакрилатного полимера в отношении клеток карциномы молочной железы человека MCF-7 позволило установить, что удельное содержание в препарате полиакрилатного полимера и его молекулярная масса практически не сказываются на биологических свойствах конечного продукта
Based on the amount of the initial reactants consumed during aurumacryl synthesis, it was concluded that the final metal wt% in the substance would be 8.03% whereas the molecular weight of the polymer would fall within the range between 100 and 300 kDA
Based on the correlation between the concentration of gold ions measured from the absorbance at 226.5 nm and the cytotoxic activity of the obtained aurumacryl samples, we developed a method for assessing the quality of the synthesized aurumacryl
Summary
Сопоставление цитотоксической активности полученных образцов аурумакрила со значительно различающимся соотношением ионов золота и полиакрилатного полимера в отношении клеток карциномы молочной железы человека MCF-7 позволило установить, что удельное содержание в препарате полиакрилатного полимера и его молекулярная масса практически не сказываются на биологических свойствах конечного продукта. Aurumacryl first described in [1] is an incomplete metal salt of poly(acrylic acid) (PAA) containing gold (III) ions. Aurumacryl was synthesized by combining aqueous PAA solutions and chloroauric acid tetrahydrate (HAuCl4) at room temperature [1]. The actual content of aurumacryl components in the resulting compound, including polyacrylic acid, gold ions (III), gold ions (I), elemental gold, and other anions and cations, cannot be deduced from the presented data. The molar ratio between AuCl–4 anions and PAA in the reaction mixture was 1 : 5 and 1 : 10
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