Abstract
A representation of the excellent versatility of poly(DVB-co-PBA) microspheres in the preparation of functional materials.
Highlights
Denaturation and aggregation of protein is the most acute problem in the utilization of protein.[1,2,3] Protein is vulnerable to environmental impact in vitro
To demonstrate the exibility of the multi-component organic particles microspheres of poly(DVB-co-PBA) in the preparation of hybrid materials, three hybrid polymer microspheres of core–shell structure were prepared in three types of emulsions by Pickering emulsion polymerization, respectively
The results indicated that the material in group of (c) had the ability to stable the conformation of protein, which could reduce the hydrophobic area of horseradish peroxidase (HRP) under heat stress denaturation
Summary
Denaturation and aggregation of protein is the most acute problem in the utilization of protein.[1,2,3] Protein is vulnerable to environmental impact in vitro. The poly(DVB-co-PBA) particles (100 mg), L-cysteine (50 mg) and AIBN (5 mg), were dispersed in the ethanol–water (2 : 3, v/v) mixture in a round-bottomed ask. The poly(DVB-co-PBA) particles (200 mg) were dissolved in 15 mL toluene as an oil phase, and ultrasound for 10 minutes. The poly(DVB-co-PBA) particles (50 mg) were dissolved in 15 mL chloroform as an oil phase, and ultrasound for 10 minutes. The poly(DVB-co-PBA) @L-Cys (20 mg) was dissolved in 3 mL distilled water (DDW) as a water phase, the initiator of benzoylperoxide (4.0 mg) was dissolved in 1.5 mL divinylbenzene as an oil phase. A er polymerization, the resultant product was ltered with a sand core funnel of G5, and the products were washed with methanol for three times to remove the residual oligomers and monomers, vacuum drying
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