Abstract
This study proposes a strategy for the rapid and simple synthesis of gold nanoparticles (CGA-AuNPs) with different particle sizes using trisodium citrate (TSC) as the first reducing agent and chlorogenic acid (CGA) as the second reducing agent. And the antibacterial activity of CGA-AuNPs with different particle sizes in vitro was checked by measuring the growth curves of Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). The CGA-AuNPs obtained by the analysis of transmission electron microscope (TEM) images and ultraviolet–visible (UV–Vis) spectra were mainly spherical, and the average diameters were 18.94 ± 1.81, 30.42 ± 6.32, 37.86 ± 3.80 and 48.72 ± 6.47 nm, respectively. High-resolution transmission electron microscopy (HRTEM) and selected area electron diffraction (SAED) showed that these nanoparticles were polycrystalline gold structures. Both CGA-AuNPs and CGA have excellent antibacterial activity, and CGA-AuNPs with small particle size has a stronger antibacterial effect than the larger one. UV–Vis absorption spectrum data revealed that the synthesized CGA-AuNPs without adding other stabilizing agent were well maintained even after 26 days. This work provides a special idea to regulate the size of CGA-AuNPs with CGA by chemical synthesis, and the potent antibacterial activity of these CGA-AuNPs may be applied in the field of antibacterial in the future.
Highlights
The vigorous development of nanotechnology has led to the large-scale development and application of nanomaterials
With the increase of the concentration of trisodium citrate (TSC) in the system, the position of the characteristic absorption peak of chlorogenic acid (CGA)-AuNPs was obviously blue-shifted, indicating that the particle size decreases with the increasing concentration of the reducing agent TSC
With the increase of the concentration of TSC, we found that the absorption value of CGA-AuNPs no longer increased and the characteristic absorption peak no longer shifted blue, indicating that almost all the gold ions in the system were reduced [28]
Summary
The vigorous development of nanotechnology has led to the large-scale development and application of nanomaterials. Some nanomaterials have been found to 2 have antibacterial functions [7], such as nano silver [8,9], graphene [10,11], carbon nanotubes [12] and nanozinc oxide [13,14] Because these nano-antibacterial materials have the advantages of a broad antibacterial spectrum and no drug resistance. There are relatively few studies using the natural compound for the synthesis and regulation of the sizes of gold nanoparticles. In this experiment, we chose CGA with antibacterial properties as one of the reducing agents for the synthesis of CGA-AuNPs with different particle sizes. Under the same particle size, the antibacterial effect of CGA-AuNPs on S. aureus (ATCC 25923) was significantly better than E. coli (ATCC 25922)
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