Abstract
Most genes in eukaryotic cells and their viruses contain non-coding intervening sequences (introns)1–5. These sequences are excised from each transcript during maturation of mRNA by a process known as RNA splicing. Although very little is known about the mechanism by which splicing occurs or its biological significance, several studies have suggested that the removal of introns is a necessary prerequisite to the accumulation of stable, cytoplasmic mRNA6–10. Here we describe a mutant of adenovirus 5 (Ad5) containing a deletion within early region Ela; the deletion prevents splicing of the normal Ela transcripts. The unspliced transcript is found in the cytoplasm of infected cells, showing that for this gene, splicing is not necessary for the biosynthesis of cytoplasmic message.
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