Abstract
Dental pulp cells were isolated from rabbit incisor by trypsin and collagenase, and cultured in monolayer. After incorporation of 14C-L-proline into pulp cells the radioactive collagen was purified and characterized by biochemical methods. The results showed that cells at fresh isolation from tissue and after culture to 30 days synthesized mainly type I collagen with type III col-lagen being a minor component. However, it was demonstrated that pulp cells cultured over 30 days began to synthesize type I trimer collagen [α1 (I)] 3 and that its content gradually increased through culture time to 60 days.
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