Synthesis of a tri‐tritiated heterobifunctional reagent, a potential tool in photoaffinity labeling technology

Abstract

AbstractThe synthesis of the radiolabelled heterobifunctional reagent N‐methyl‐N‐chloroformyl‐N′‐terbutoxycarbonyl‐para‐phenylenediamine A (fig. 1) is presented. In particular, the use of tri‐tritiated methyl iodide in defined reaction conditions allowed the synthesis of this probe B (fig. 1) with a high specific radioactivity (77 Ci/mmol).