Synthesis of a copolymer containing adenylic and deoxyadenylic acid residues with polynucleotide phosphorylase

Abstract

We report here the synthesis and characterization of copolymers containing both AMP and dAMP residues using polynucleotide phosphorylase from Micrococcus luteus . The copolymerization proceeds most readily when Mn 2+ replaces the usual Mg 2+ in the reaction. The dAMP residues occupy internal positions in the polymer chain, and the chains are several hundred nucleotide residues long.