Synthesis of 6-(2-Methoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione as a New Reagent for Spectrophotometric Determination of Copper

Maliheh Barazandeh Tehrani,Hutan Ghanbari,Effat Souri,Fazel Shamsa,Mohsen Amini

doi:10.1155/2014/260179

Abstract

A simple, sensitive, accurate, and green spectrophotometric method for the determination of Cu(II) using newly synthesized reagent, 6-(2-methoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione (MNDTT), has been developed. MNDTT was synthesized based on the acylation of methoxy naphthalene and reaction of the product with amyl nitrite, which upon reaction with thiosemicarbazide yielded 6-(2-meyhoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione. MNDTT produces a dark red complex with copper in methanol according to the 1 : 2 stoichiometry. Beer's law was obeyed over the concentration range of 2.5–20 µg/mL with r 2 = 0.992. The limit of detection and limit of quantification were 0.33 and 1.10 µg/mL, respectively. Within-day and between-day precision values were less than 3.68%. Finally, the method has been applied to a dental alloy (110-plus) successfully and the results were compared with atomic absorption method. The results showed that there was no significant difference between the two methods (P > 0.05).

Highlights

Copper is an essential micronutrient for many vital processes
Copper is present in a large number of enzymes, which are involved in electron transfer, activation of oxygen, and other small molecules as well as superoxide dismutation [1, 2]. It serves as an essential cofactor for a variety of proteins involved in neurotransmitter synthesis as well as in neuroprotection via the Cu/Zn superoxide dismutase
In a series of 5 mL volumetric flask, 1 mL (2.5–20 μg/mL) of Cu(II) and 2 mL of MNDTT (0.002 M) were taken and the volume was adjusted to 5 mL with methanol

Summary

Introduction

Copper is present in a large number of enzymes, which are involved in electron transfer, activation of oxygen, and other small molecules as well as superoxide dismutation [1, 2]. It serves as an essential cofactor for a variety of proteins involved in neurotransmitter synthesis as well as in neuroprotection via the Cu/Zn superoxide dismutase. Some methods use organic solvents such as chloroform or acetonitrile which are for the issue of environmental concern or worldwide storage crisis Among these techniques, visible absorption spectrophotometry represents the most convenient technique because of the availability of the instrumentation, simplicity, speed, precision, accuracy, and low cost. In this investigation the synthesis of 6-(2-methoxynaphthyl)-2,3-dihydro-1,2,4-triazine3-thione has been synthesized as a chromogenic reagent for the determination of Cu(II) and validation of the developed method is reported

Experimental

Results and Discussion

Validation of the Method

Conclusion