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Abstract
The conversion of 2-ketoglutarate-[ 14C] to 5-aminolevulinic acid-[ 14C] (ALA) by a cell-free system from maize leaves is described. Optimal conversion was achieved at pH 6.2 using a 20 000 g supernatant after gel filtration through Sephadex G-25. The formation of ALA required Mg 2+, an amino donor (alanine or glutamate), pyridoxal phosphate and NADH. NADPH was somewhat less effective.
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