Abstract
The 2-aminoethyl β-glycoside of the trisaccharide 3′-(N-glycolylneuraminyl)lactose was synthesized in the framework of studies of approaches to the preparation of synthetic tumor vaccines based on carbohydrate chains of tumor-associated gangliosides and converted to the corresponding 2-4-maleimidobutanoylamino)ethyl glycoside. The reaction of the latter with thiolated hemocyanin from the keyhole limpet Megathura crenulata (KLH) gave a carbo-hydrate—protein conjugate, a tumor vaccine prototype containing about 250 trisaccharide ligands attached to a macromolecular KLH complex. A polyvalent conjugate with a polyacryl-amide support was synthesized to be used as a coating antigen in the control of specificity and efficiency of immune response by enzyme-linked immunosorbent assay (ELISA).
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