Abstract
Purpose: To study the anti-ulcer activity of 2’-(5-Chloro-2-hydroxybenzylidene) benzenesulfanohydrazide Schiff base against ethanol-induced gastric mucosal lesions in rats. Methods: 2’-(5-Chloro-2-hydroxybenzylidene) benzenesulfano-hydrazide (Cl-BzSO-HAP) was synthesized, and structurally characterized by elemental analysis, FT-IR, 1 H-NMR and C-NMR. Its preventive activity against ulcer induced by absolute ethanol in Sprague-Dawley rats were studied in vivo. Twenty four Sprague Dawley (SD) rats (12 males and 12 females) were assigned equally into 4 groups (n = 6), including negative control and positive control groups. The third group received a low oral dose of the compound (50 mg/kg) while the fourth group was administered a high oral dose (100 mg/kg). The degree of gastric lesion formation was assessed. Prior to dosing, the animals were fasted overnight. Results: Cl-BzSO-HAP showed significant preventive activity against ulcer induced by absolute ethanol. Oral administration of the compound (both low and high doses) prior to ethanol administration inhibited gastric lesion formation by 88.0 and 94.9 %, respectively, compared to 82.7 % for the positive control, cimetidine. Conclusion: Extensive gastric necrosis in rats induced by absolute ethanol was prevented by administration of Cl-BzSO-HAP resulting in reduced or total absence of lesions. Keywords: Benzenesulfanohydrazone, Schiff base, Gastric ulcer, Histology.
Highlights
Ulcer is an open sore, or lesion, usually found on the skin or mucous membranes of the body
This study extends this work by synthesizing a different Schiff base and evaluating its anti-ulcer activity
Animal experiments were performed in accordance with the guidelines for animal experimentation issued by the Animal Care and Use Committee at the University of Malaya (Ethics no.: PM/27/07/2010/MAA)
Summary
Ulcer is an open sore, or lesion, usually found on the skin or mucous membranes of the body. Researchers determined that stomach fluid compounds – hydrochloric acid and pepsin – contribute to ulcer formation. All the three factors – lifestyle, acid and pepsin, and H. pylori – play a role in ulcer development, H. pylori is considered to be the primary cause. This study extends this work by synthesizing a different Schiff base and evaluating its anti-ulcer activity. The chemicals, 5’-chloro-2’-hydroxyacetophenone, benzenesulfanohydrazide and Approximately 1 - 10 mg of the compound was weighed accurately on a microbalance, and combusted at high temperatures in a stream of oxygen during which the products of combustion (carbon, hydrogen, and nitrogen) were recorded in a single analysis. Between 1 and 3 mg of ground Schiff base crystal was mixed thoroughly with about 350 mg of ground KBr. The mixture was transferred to a die that has a barrel diameter of 13 mm.
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