Abstract
The procedure described utilizes a crude cell-free extract from the yeast Saccharomyces cerevisiae as enzymatic source for the synthesis of coproporphyrin III from [ 14C]δ-aminolevulinic acid with a high yield of conversion (⋍60%). Both specific radioactivity and total radioactivity of coproporphyrin III can be adjusted fairly well. This procedure is not time consuming for yeast acellular extracts or porphyrin ester preparations. The acellular extracts can be stored frozen (−30°C) for at least 1 year without loss of enzymatic activity. The same procedure can be used for [ 14C]protoporphyrin preparation.
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