Synthesis of [14C]‐ and [35S]‐labelled PI‐88 for pharmacokinetic and tissue distribution studies

Abstract

AbstractPI‐88, uniformly labelled with [14C] was prepared by incorporating D‐[14C]glucose into the fermentation of Pichia (Hansenula) holstii NRRL Y‐2448 under controlled conditions to produce [14C]‐labelled extracellular phosphomannan. Subsequent acid catalyzed hydrolysis gave the [14C]‐labelled oligosaccharide phosphate fraction which was sulfonated with excess sulfur trioxide pyridine complex to give [14C]PI‐88. [35S]‐labelled PI‐88 was similarly prepared by the sulfonation of unlabelled oligosaccharide phosphate fraction with [35S] sulfur trioxide pyridine complex. Copyright © 2002 John Wiley & Sons, Ltd.