Synthesis of α - mangostin-D-glucoside in supercritical carbon dioxide media.

Abstract

α-Mangostin, the major xanthone constituent of mangoteen fruit pericarp, has several important pharmaceutical application but its bioavailability is restricted due to its insolubility in water. Herein, we synthesized water soluble α-mangostin-D-glucoside by glycosylation of α-mangostin at hydroxyl group; using amyloglucosidase (3.2.1.3) catalyzed reaction in supercritical carbon dioxide (SC-CO2) media. Response surface methodology (RSM) based on a five-variable central composite rotatable design involving 32 experiments was used to determine the effect of pressure (80-160bar), temperature (35-75°C), enzyme concentration (15-45mg), buffer pH (4.0-8.0) and buffer volume (1.0-5.0mL). Experimental data fitted the second-order polynomial equation as indicated by R(2) value of 0.94. The optimal enzymatic conversion within the experimental range of the variables reached 20.3% at a pressure of 120bar, temperature of 55°C, enzyme concentration of 30mg, buffer volume of 3mL and pH6.0 which is well matched with the predictive yield.