Synthesis, In-Silico Potential Enzymatic Target Predictions, Pharmacokinetics, Toxicity, Anti-Microbial and Anti-Inflammatory Studies of Bis-(2-methylindolyl) Methane Derivatives

Abstract

Background: The bis(indolyl)methanes (BIMs) scaffold is reported for wide varieties of pharmacological profiles, including antibacterial, anti-proliferative, anticancer, cytotoxic, insecticidal, analgesic, antioxidant, and anti-inflammatory agents. Materials and Methods: A series of bis(indolyl)methanes have been synthesized by a greener and newer approach using the reaction of different substituted aldehydes and indole in the presence of an easily available and biodegradable base such as piperidine in acetic acid at room temperature and characterized with ultraviolet–visible spectrophotometry (UV–Vis or UV/Vis), Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), etc. The antibacterial and antifungal activities were also carried out against Staphylococcus aureus (RCMB 000106) and Bacillis subtilis (RCMB 000107), as two Gram-positive bacterial strains, and Salmonella typhi and Escherichia coli (RCMB 000103), as two Gram-negative bacterial strains. Fungal species such as Candida Albicans, Penicillium chrysogenum, Aspergillus niger were also used for in vitro antifungal evaluation. All our newly synthesized 14 compounds (4a-4n) were subjected for anti-inflammatory activity in vitro and compared with the known standard drug aceclofenac. Results: Our newly synthesized compounds showed good to moderate antibacterial agents, in silico ADMET, and anti-inflammatory profiles. Conclusion: We hope that our current study would aid future developments of bis(indolyl)methanes as antibacterial and anti-inflammatory agents.