Synthesis, Crystal Structures and Anti-lung Cancer Effects of Two Copper(II) Coordination Polymers Mediated by Mitochondrial-Dependent Apoptosis

Abstract

In this study, two new Cu(II)-based coordination polymers {[Cu2(pdc)2](DMF)2}n (1, H2pdc = pyridine-3,5-bis(phenyl-4-carboxylic) acid) and {[Cu2(ndc)2(bpee)2]2.5H2O}n (2, H2ndc = 1,2-naphthalenedicarboxylic acid, bpee = trans-1,2-bis(4-pyridyl)ethylene) have been successfully prepared under different solvothermal conditions. In addition, the anti-proliferation activity of compounds 1 and 2 was detected against the human non-small cell lung cancer NCI-H1650 with MTT assay. The cell viability curves and compounds IC50 values indicated that both of these two new synthesized compounds have anti-proliferation activity on NCI-H1650 cells, and compound 1 showed stronger inhibitory effect than compound 2. And then, the LDH detection and Annexin V-FITC/PI assay was used to explore the manner of NCI-H1650 cell death. The intracellular ATP and ROS level was measured to confirm whether the apoptotic cell death was caused in a mitochondrial-dependent manner.