Synthesis, characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Rashed Taleb Rasheed,Thaer A Abdulla,Noor Al-Jammal,Endre Domokos,Ali Dawood Salman,Hadeel S Mansoor,Thamer Adnan Abdullah,Tatjana Juzsakova,Rasha R Al-Shaikhly,Phuoc Cuog Le

doi:10.1007/s10973-020-09725-5

Abstract

This paper deals with an advanced colorimetric method used to determine the catalase mimetic activity of V2O5 nanoparticles by measuring the decrease in potassium permanganate concentration in a mixture containing V2O5 and hydrogen peroxide. The experiments were carried out in batch reactor at room temperature for 3 min at wavelength number of 525 nm. Vanadium pentoxide was synthesized by hydrothermal method (reflux) from ammonium metavanadate (NH4VO3) as a precursor and cetyltrimethylammonium bromide as a surfactant. The annealing of the product was carried out for 2 h, at temperatures of 250, 500 and 750 °C. In order to determine the structure and the chemical nature of the nanoparticles prepared, the characterization was carried out by X-ray diffraction and scanning electron microscopic techniques. Atomic force microscopic and thermal gravimetric investigations have shown the decomposition steps of V2O5 at different temperatures. UV–visible spectroscopic technique and Fourier transform spectrometry were used to further characterize the nanoparticles. Advanced colorimetric method was used to study the catalase mimetic activity of the newly synthesized vanadium pentoxide (V2O5) nanoparticles using hydrogen peroxide (H2O2) as substrate. V2O5 nanoparticles resulted in an increase in the catalase mimetic activity with increasing the annealing temperature of the V2O5 nanoparticles. The maximum activity was found at 500 °C, which subsequently decreased with further increase in the annealing temperature.

Highlights

Hydrogen peroxide is a by-product produced inside of the cells of living organisms and has detrimental effects on them
Advanced colorimetric method was devised and used first time to determine the catalase mimetic activity of V2O5 nanoparticles using potassium permanganate solution as indicator, instead of the commonly used UV–visible spectrophotometry (UV–Vis) method which is used by the researchers
Vanadium pentoxide nanoparticles were prepared by hydrothermal method, and the nanoparticles were annealed at different temperatures (250, 500 and 750 °C) for 120 min

Summary

Introduction

Hydrogen peroxide is a by-product produced inside of the cells of living organisms and has detrimental effects on them. Catalases are enzymes used by the cells to get rid of hydrogen peroxide and protecting them from its harmful impacts. Extracting the catalase enzyme from the cells and using it as a medication is costly, and by introducing the enzyme into the human body by injection or ingestion, it will not penetrate into the cells because of its high molecular mass. It will function outside the cells and will have a minor effect. As a result, utilizing compounds that mimetic the enzymatic function of catalase is a welcome method by the researchers

Methods

Results

Conclusion