Synthesis, characterization, DNA interaction, thermal and in vitro biological activity investigation of some Ni(II)-Isatin bishydrazone complexes

Abstract

Complexes of the type [Ni (L) (H2O)] Cl2.nH2O, where L = [(pyridine-2-carboxaldhyde)-3-isatin]-bishydrazone (cpish), [(2-acetyl pyridine)-3-isatin]-bishydrazone (apish) and [(2-benzoyl pyridine)-3-isatin]-bishydrazone (bpish) have been synthesized and characterized on the bases of elemental analysis, molar conductance, IR, NMR, electronic spectra and thermal analysis (TGA and DTA). Moreover, the stoichiometry and the formation constants of these complexes have been determined spectrophotometrically. Kinetics and thermodynamic parameters of the thermal decomposition have been computed from the thermal data using Coats and Redfern method, which confirm first-order kinetics. The bioefficacy of the ligands and their complexes have been examined for their in vitro antibacterial and antifungal activity against many types of bacteria and anti fungal cultures, which are common contaminants of the environment in Egypt, and the results indicate that the ligands and their metal complexes possess notable antimicrobial activity. Investigation of their interaction with CT-DNA under physiological conditions, using spectroscopic (UV–visible) and hydrodynamic techniques (viscosity measurements). Binding constant "K b" obtained from spectroscopic methods revealed significant binding of compounds with DNA via intercalation, Furthermore, free energies of compounds–DNA interactions indicated spontaneity of their binding.