Abstract
In this study tagging of 99mTc with PZN was examined with regards to different concentration of PZN, stannous chloride, 99mTc and pH ranges from 5.00-6.00. The suitability of the 99mTc labeled PZN was assessed in terms of percent radiochemical purity (%RCP) yield in normal saline at concentration value of the stannous chloride as 120 mg, stability in serum (in vitro) at 37°C at different intervals, in vitro up take by live and heat killed E. coli, biodistribution behavior in artificially E. coli (live and heat killed) infected animal model rat and scintigraphic accuracy in artificially E. coli infected animal model rabbit. Maximum %RCP yields of 98.25 ± 0.26% was observed after 30 m of labeling using 2 mg of PZN, 125 μg stannous chloride, 2.5 m Ci sodium pertechnetate and pH 5.3. The labeled PZN showed stable profile in serum at 37°C with a total decay of 16.40 ± 0.18%. The labeled PZN showed 72.55 ± 0.90% maximum bacterial uptake after 90 min in live and in case of heat killed E. coli, no significant uptake was seen. The labeled PZN showed 14.75 ± 0.40% absorption and accumulation in the infected muscle of the rat infected with live E. coli and infected to normal muscle ratio was 6:1. Scintigraphically, it was observed that after few minutes, the activity accumulated in the infected muscle was more than the others sites. The instant investigation substantiated that labeling PZN through this technique as compared to 99mTcV:N~ and 99mTc(CO)3~ showed almost analogous %RCP yield, stability in serum at 37°C, in vitro binding with E. coli, biodistribution profile in animal modal rats and scintigraphic accuracy in animal model rabbit.
Highlights
In time diagnosis of infection and its discrimination from noninfectious site is challenging for clinicians but imperative for decisive planning and suitable treatment
Detection and monitoring of a variety of infectious diseases can be accomplished using a range of diagnostic modalities like ultra-Sound (US), Computed Tomography (CT), Magnetic Resonance Imaging (MRI), etc
The PZN labeled with 99mTc using different concentration of PZN, 99mTc, stannous chloride at different pH showed different percent radiochemical purity yield
Summary
In time diagnosis of infection and its discrimination from noninfectious site is challenging for clinicians but imperative for decisive planning and suitable treatment. These investigative modalities have proven helpful in recognizing infections because of their exceptional resolution, which facilitate the clinicians to evaluate more precisely the internal sites of indistinct infection [4]. These investigative modalities depend only on morphological alterations and most anomalies can only be detect at complex stages of ailment and diagnosis of in time infections and its discrimination from inflammation or other involvements can be tricky [5]. Nuclear medicine (NM) procedures in contrast to US, CT and MRI, can find out the site and degree of infection on the basis of physiologic and metabolic alterations [6]
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