Abstract
Background: Selenium is an important trace mineral required by the animals. It is an integral part of antioxidant system, protecting the body against free radical injury. Nano particles attract a widespread attention due to its high bioavailability and efficacy. The current study was aimed to synthesize, characterize nano selenium and evaluate the cytotoxic effect of nano selenium and organic selenium (selenocysteine) under in vitro condition in vero cell line. Methods: Nano selenium was synthesized by wet chemical method by using sodium selenite, selenium powder, ascorbic acid and sodium hydroxide at laboratory level. In this study particle size, shape, zeta potential and selenium content were characterized by using Particle Size Analyser (PSA), Transmission Electron Microscope (TEM) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The toxicity was analysed by MTT assay against vero cell line. Result: The result revealed that selenium nano particles were spherical in shape, nano in size (less than 50 nm) and pure in nature. The nano selenium and organic selenium (selenocysteine) effectively inhibited the growth of vero cells in a dose dependent manner.
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