Synthesis, characterization and phosphotriesterase mimetic activity of some Zn(II) and Cu(II) complexes

Abstract

We report here the synthesis and characterization of a few phenolate-based ligands bearing tert- amino substituent and their Zn(II) and Cu(II) metal complexes. Three mono/binuclear Zn(II) and Cu(II) complexes [Zn(L1)(H2O)].CH3OH.H2O (1) (H 2 L1 = 6,6′-(((2-dimethylamino)ethylazanediyl)bis(methylene))bis(2, 4-dimethylphenol), [Zn2(L2)2] (2) (H 2 L2 = 2,2′-(((2-dimethylamino)ethyl)azanediyl)bis(methylene)bis(4- methylphenol) and [Cu2(L3)2.CH2 Cl2] (3) (H 2 L3 = (6,6′-(((2-(diethylamino)ethyl)azanediyl)bis(methylene)) bis(methylene))bis(2,4-dimethylphenol) were synthesized by using three symmetrical tetradendate ligands containing N2O2 donor sites. These complexes are characterized by a variety of techniques including; elemental analysis, mass spectrometry, 1H, 13C NMR spectroscopic and single crystal X-ray analysis. The new complexes have been tested for the phosphotriesterase (PTE) activity with the help of 31P NMR spectroscopy. The 31P NMR studies show that mononuclear complex [Zn(L1)(H2O)].CH3OH.H2O (1) can hydrolyse the phosphotriester i.e., p-nitrophenyl diphenylphosphate (PNPDPP), more efficiently than the binuclear complexes [Zn2(L2)2] (2) and [Cu2(L3)2.CH2Cl2] (3). The mononuclear Zn(II) complex (1) having one coordinated water molecule exhibits significant PTE activity which may be due to the generation of a Zn(II)-bound hydroxide ion during the hydrolysis reactions in CHES buffer at pH 9.0. Synthesis and phosphotriesterase (PTE) activity of three Zn(II) and Cu(II) complexes based on bis-phenolate ligands are described. The structures of these complexes were studied by spectroscopic and single crystal X-ray techniques. The mononuclear Zn(II) complex having coordinated water ligands exhibited high PTE activity as compared to the binuclear Zn(II) and Cu(II) complexes.