Synthesis, characterization and cytotoxic studies of novel 1,2,4-triazole-azomethine conjugates

Abstract

A series of 1,2,4-triazole-schiff hybrids were synthesized and characterized by mass spectrometry, FTIR and NMR spectroscopy. The compounds were screened for anticancer activity against human breast cancer cell line (MCF-7 and T47D) and human cervical cancer cell line (HeLa). The result indicates that newly synthesized compounds exhibit cytotoxicity to all cell lines studied. In particular, MCF-7 cells were shown to be more sensitive with EC50 < 50 μM/ml with the exception of compound T2. Interestingly, T6 produced more high cytotoxicity on MCF-7 cells (< 10 μM/ml), which is comparable to cisplatin EC50 13.10 μM/ml. To further investigate the mode of cell death, early and late apoptosis studies were done on MCF-7 cells. The externalization of phosphatidylserine and DNA fragmentation supports the apoptosis as the major mode of cell death induced by derivatives on MCF-7 cells.