Synthesis, Characterization and Biodegradability of Poly(Ethylene Glycol)-Bound Molecule Platinum Complexcontaining Ferrocenyl Moiety Using MALDI Time-of-Flight Mass Spectrometry

Abstract

Monomethoxypolyethylene glycol (MPEG) was bound to a ferroceny/ketimine and the resulting molecule complexed with platinum ion. The chief means of characterization of the intermediate and final compounds was Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry. The results indicated some degree of decomposition by the MALDI source for the intermediate compounds and the presence of platinum complex as a chelate. The biodegradability of the MPEG-bound molecule complex was also studied using papain at 37°C and pH 7.80 in ammonium phosphate buffer to avoid metal catonization during mass ionization. The mass spectra showed that the complex was 50% degraded within 20 min and then remained unchanged up to 48 hours.