Synthesis, characterization and antifungal activity of biosynthesized silver nanoparticle

Abstract

In this experiment an effort was made to synthesize silver nanoparticle biologically from Trichoderma asperellum, a potential indigenous biocontrol agent. Silver nitrate was added as precursor for the synthesis of silver nanoparticle. The biosynthesized silver nanoparticle was characterized by UV–Vis spectrophotometer, dynamic light scattering (DLS), X-ray diffraction (XRD), zeta potential and transmission electron microscope (TEM). UV Vis spectrum of aqueous medium containing silver ion showed peak at a wavelength of 420 nm corresponding Plasmon Absortion of silver nanoparticle. DLS study showed that the biosynthesized silver nanoparticles have a size of 27.64 nm with polydispersity index (PDI) of 0.409. The charge of silver nanoparticle was determined by zeta sizer and found to have negative potential value of − 1.34. It indicates that the biosynthesized nanoparticle are stable on dispersion. TEM study revealed the formation well dispersed silver nanoparticle in the range of 9–41 nm with roughly spherical in shape. Fungicidal activity of silver nanoparticle at different concentration (100 ppm, 50 ppm, 30 ppm, and 10 ppm) tested against four soil borne plant pathogens viz., Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, and Sclerotium rolfsii and comparison was made with Carbendazim @3000 ppm. The result showed that the silver nanoparticle at 100 ppm showed significantly higher efficacy in inhibiting mycelial growth of the pathogens as compared to the Carbendazim at 3000 ppm.