Synthesis, characterization, and anticancer properties of Ag nanoparticles derived from walnut leaves tested on cells of L929, MCF-7 and H1299

Abstract

In this study, Ag nanoparticles (Ag NPs) were synthesized using walnut leaves according to a straightforward method. The synthesized Ag NPs were characterized by advanced analytical techniques such as XRD, TEM, UV–Vis, FESEM, EDS and FTIR. The synthesized Ag NPs tested on some cells to detect its antiproliferative effects. The antiproliferative effects of Ag NPs derived from walnut leaves were investigated on L929, MCF-7 and H1299 cells. The characterization studies showed that Ag NPs were well formed and the cell studies revealed that the synthesized Ag NPs have considerable anticancer effects. The mean particle size of Ag NPs was found to be 18.76 nm by TEM analyses. FESEM analyses revealed that Ag NPs contained Ag, C, and O elements and FTIR analyses revealed these elements bound to each other with C–O bounds. The amount composition of Ag NPs was detected to be 85 % by EDS analyses. The antiproliferative effect of AgNPs obtained from walnut leaf extract on human breast cancer cell line (MCF-7), human lung cancer cell line (H1299) and mouse fibroblast cell line (L929) was determined by MTT cell viability assay method. While the silver nanoparticles synthesized within the scope of the study did not show any toxic effect on the mouse fibroblast cell line (L929) that we used for control purposes, it was observed that it was highly effective against MCF-7 and H1299 cells. Apoptosis and necrosis index studies were calculated by cell counting from randomly selected areas and these studies revealed that the synthesized silver nanoparticles suppressed cell viability by inducing apoptosis in cells. Significantly suppressing the viability of cancer cells proves that silver nanoparticles obtained from walnut leaves can be a new treatment approach for cancer treat.