Abstract
ABSTRACT. Cholesteryl- p-[ 18F]fluorobenzoate ([ 18F]CFB) was investigated as a potential adrenal positron emission tomography (PET) imaging agent for the diagnostic imaging of adrenal disorders. We describe the synthesis, biodistribution, adrenal autoradiography, and baboon PET imaging of [ 18F]CFB. The synthesis of [ 18F]CFB was facilitated by the use of a specially designed microwave cavity that was instrumental in effecting 70–83% incorporation of fluorine-18 in 60 s via [ 18F]fluoro-for-nitro exchange. Tissue distribution studies in mature female Sprague–Dawley rats showed good accumulation of [ 18F]CFB in the steroid-secreting tissues, adrenals and ovaries, at 1 h postinjection. The effectiveness of [ 18F]CFB to accumulate in diseased adrenals was shown through biodistribution studies in hypolipidemic rats, which showed a greater than threefold increase in adrenal uptake at 1 h and increased adrenal/liver and adrenal/kidney ratios. Analysis of the metabolites at 1 h in the blood, adrenals, spleen, and ovaries of hypolipidemic and control rats showed the intact tracer representing greater than 86%, 93%, 92%, and 82% of the accumulated activity, respectively. [ 18F]CFB was confirmed to selectively accumulate in the adrenal cortex versus the adrenal medulla by autoradiography. Normal baboon PET imaging with [ 18F]CFB effectively showed adrenal localization as early as 15 min after injection of the tracer, with enhanced adrenal contrast seen at 60–70 min. These results suggest that [ 18F]CFB may be useful as an adrenal PET imaging agent for assessing adrenal disorders.
Published Version
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